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Part 6: Spectroscopy

22 Infrared (IR) Spectroscopy

Radiation of lower energies causes less energetic transitions between energy levels. For example, infrared radiation is of the same order of energy as the vibrational energy levels of a molecule (which are also quantized); these are called vibrational transitions.

Infrared (IR) Spectroscopy

Infrared spectroscopy works similarly to UV-visible spectroscopy (in that it involves absorption of light) but provides different information about the sample. Electromagnetic radiation in the infrared range corresponds to the energy of molecular vibrations. Vibrational energy levels are quantized just as electronic energy levels are. It is possible to measure the absorption of the IR radiation as it causes the molecule to change from one vibrational energy level to another.

This image is the chemical structure of cyclohex-2-en-1-one.

A typical IR spectrum appears to be quite complicated, and a great deal of information can be obtained about the structure of the compound under investigation. In organic chemistry, the IR spectrum is very useful because particular functional groups have very specific frequencies at which they absorb. For example, carbonyl groups typically show absorptions in the region between 1800 and 1620 cm-1. The units on a typical IR spectrum are expressed in wavenumbers (cm-1) which is the reciprocal of the wavelength of radiation absorbed.

FTIR spectrum of cyclohex-2-en-1-one showing peaks at approximately 3000-3100 cm^-1 (C-H stretching), 1650-1750 cm^-1 (C=O stretching), and 1000-1300 cm^-1 (C-C and C-H bending).
Figure 17. FTIR spectrum of cyclohex-2-en-1-one.

Figure 17 is a transmission IR spectrum of cyclohex-2-en-1-one (NIST WebBook). The most prominent feature is the strong absorption (i.e., little to no transmission) at about 1700 cm-1 which corresponds to the carbonyl (C=O) stretching frequency. You may also notice the peaks slightly above 3000 cm-1 to be sp2 hybridized carbon to hydrogen (Csp2-H) stretching, while peaks slightly below 3000 cm-1 to be sp3 hybridized carbon to hydrogen (Csp3-H) stretching.

The following table lists some common infrared spectroscopy absorptions by frequency regions.

Peak Position (cm-1) Group Class Peak Details
3200-3550 O-H stretching alcohol strong, broad
3500-3350 N-H stretching amine medium
2500-3300 O-H stretching carboxylic acid strong, broad
2700-3200 O-H stretching alcohol weak, broad
3267-3333 C-H stretching alkyne strong, sharp
3000-3100 Csp2-H stretching alkene medium
2840-3000 Csp3-H stretching alkane medium
2222-2260 C≡N stretching nitrile weak
1818-1650 C=O stretching Carbonyl containing compounds strong

For a complete list of IR spectroscopy absorptions, see Infrared Spectroscopy Absorption Table.

Collecting an IR Spectrum

The Organic Chemistry Labs at MSU use commercial infrared spectrometers, which may be controlled via a graphical interface on a tablet computer. You should only operate the IR spectrometers after receiving proper training from your TA. However, you may find it helpful to familiarize yourself with the information here so that you can better follow along when it comes time to take IR spectra.

Application Startup and Spectrum Collection

The software has instruction screens built into it. The following instructions provide a detailed walkthrough and additional notes.

  1. Sprinkle some ethanol on a Kimwipe. Clean the round panel using Kimwipes and ethanol. Do not sprinkle ethanol directly onto the FTIR instrument.
  2. Start OMNIC Paradigm software on the instrument screen. Wait till the signal on the top right indicates “Nicolet Summit”.
  3. Click “Background” – wait for preview – click “Start Background Measurement”. This process may take 30 seconds.
  4. Load a SMALL amount of solid sample on the center of the round panel—twist the top detector part down until it is loosely in contact with the solid (do not push it too tight!). If your sample is a liquid, no need to twist the top detector down.
  5. Click “Measure Sample”. This process may take 30-60 seconds.
  6. Enter a name “sec 2 gr 2 pj 2 cat 4”, for example, for your sample – click “OK”.
  7. Select and unselect from the right graph list to only show your spectrum. Eye button enabled: show spectrum. Eye button disabled: hide spectrum.
  8. Click “File”→“Create Report”→“Create”→“Print”→Save pdf to your USB drive (inserted next to the screen). Eject the USB drive after the file was saved.
  9. Clean bottom panel and top detector with Kimiwipes and ethanol.
  10. Perform a simple analysis on the spectrum you collected. Ask your TA for assistance if you have any questions.

In conclusion, FTIR spectroscopy is a powerful analytical technique that provides valuable information about the chemical composition and structure of a wide range of materials. By understanding the principles behind FTIR and mastering the techniques involved, you will be able to analyze unknown samples, identify functional groups, and monitor chemical reactions with confidence.

Part of this chapter has been adapted from the CEM 161/162 manual: Cooper, M. M. et. al. Cooperative Chemistry for Michigan State General Chemistry Laboratories, 2019.

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Cooperative Organic Chemistry Student Laboratory Manual Copyright © 2025 by Elizabeth L. Day; Melanie M. Cooper; and Mengqi Zhang is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, except where otherwise noted.